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Thermo Scientific™ Pierce™ Rat IFN gamma ELISA Kits


Measure levels of rat interferon gamma (IFNg) in rat serum, plasma (EDTA, heparin, sodium citrate), and culture supernatant samples using 96-well plates.

Manufacturer: thermo scientific™ pierce™  ERIFNG

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Catalog No. ENERIFNG

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Description & Specifications

Specifications

Assay Range 8 to 500pg/mL
Description Rat IFN gamma ELISA Kit
Format Colorimetric ELISA (TMB substrate) in clear 96-well strip plate
Formulation Complete ELISA kit with coated 96-well plate
Kit Contents 30X Wash Buffer, 50mL
Measurement Absorbance at 450nm minus absorbance at 550nm
Quantity 1 plate kit
Sensitivity <2pg/mL
Sample Type Cell culture supernatant, serum, plasma (EDTA, sodium citrate and heparin)
Sample Size 50µL
Specificity No significant cross-reaction with rat IL-1a, IL-1ß, IL-2, IL-4, IL-6, IL-10, MIP-1a, RANTES, or TNFa, or human or pig IFNg. When preparations of NIH/NIBSC mouse IFNg (lot Gg02-901-533) were evaluated in the Rat IFNg ELISA Kit, 6250pg/mL mouse IFNg was eq
Sufficient For 96 microplate wells
Total Assay Time 2.5 hr.
Target Natural and recombinant rat interferon gamma (IFNg)

Assay IFN-γ(rat interferon gamma) levels in rat serum, plasma (EDTA, heparin, sodium citrate), and culture supernatant samples with our Thermo Scientific Rat IFN gamma ELISA Kits.

These rat IFN-gamma ELISA kits use pre-titered, matched pairs of coating and detection antibodies to achieve sensitive and accurate measurement of interferon gamma (IFNγ) in samples at 50 per well. Also included are calibrated recombinant protein standard, streptavidin-HRP, TMB substrate solution and stop solution. The complete kits contain precoated 96-well strip plate(s) and lot-optimized reagents. All components undergo quality control testing to minimize lot-to-lot variability.

  • Target: Natural and recombinant rat interferon gamma (IFNγ)

  • Format: Colorimetric ELISA (TMB substrate) in clear 96-well strip plate

  • Measurement: Absorbance at 450nm minus absorbance at 550nm

  • Assay Range: 8 to 500pg/mL

  • Sensitivity: < 2pg/mL

  • Sample Types: Cell culture supernatant, serum, plasma (EDTA, sodium citrate and heparin)

  • Total Assay Time: 2.5 hours

  • Sample Size: 50μL

  • Specificity: No significant cross-reaction with rat IL-1α, IL-1β, IL-2, IL-4, IL-6, IL-10, MIP-1α, RANTES, or TNFα, or human or pig IFNγ. When preparations of NIH/NIBSC mouse IFNγ (lot Gg02-901-533) were evaluated in the Rat IFNγ ELISA Kit, 6250pg/mL mouse IFNγ was equivalent to 258pg/mL rat IFNγ (4.1% cross-reactivity)

Includes:Complete kits contain pre-coated antibody-plates, detection antibodies, buffers, diluents, standards, and substrates.