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Invitrogen™ Rat IFN gamma ELISA Kits DFS Item

Measure levels of rat interferon gamma (IFNg) in rat serum, plasma (EDTA, heparin, sodium citrate), and culture supernatant samples using 96-well plates.

Manufacturer:  Invitrogen™ LSERIFNG*

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Catalog No. ENERIFNG



Description

Description

Assay IFN-γ(rat interferon gamma) levels in rat serum, plasma (EDTA, heparin, sodium citrate), and culture supernatant samples with our Thermo Scientific Rat IFN gamma ELISA Kits.

These rat IFN-gamma ELISA kits use pre-titered, matched pairs of coating and detection antibodies to achieve sensitive and accurate measurement of interferon gamma (IFNγ) in samples at 50 per well. Also included are calibrated recombinant protein standard, streptavidin-HRP, TMB substrate solution and stop solution. The complete kits contain precoated 96-well strip plate(s) and lot-optimized reagents. All components undergo quality control testing to minimize lot-to-lot variability.
  • Target: Natural and recombinant rat interferon gamma (IFNγ)

  • Format: Colorimetric ELISA (TMB substrate) in clear 96-well strip plate

  • Measurement: Absorbance at 450nm minus absorbance at 550nm

  • Assay Range: 8 to 500pg/mL

  • Sensitivity: < 2pg/mL

  • Sample Types: Cell culture supernatant, serum, plasma (EDTA, sodium citrate and heparin)

  • Total Assay Time: 2.5 hours

  • Sample Size: 50μL

  • Specificity: No significant cross-reaction with rat IL-1α, IL-1β, IL-2, IL-4, IL-6, IL-10, MIP-1α, RANTES, or TNFα, or human or pig IFNγ. When preparations of NIH/NIBSC mouse IFNγ (lot Gg02-901-533) were evaluated in the Rat IFNγ ELISA Kit, 6250pg/mL mouse IFNγ was equivalent to 258pg/mL rat IFNγ (4.1% cross-reactivity)

Includes:Complete kits contain pre-coated antibody-plates, detection antibodies, buffers, diluents, standards, and substrates.

Specifications

Specifications

8 to 500pg/mL
Colorimetric ELISA (TMB substrate) in clear 96-well strip plate
HRP Dilution Buffer, 13mL, TMB Substrate Solution, 12mL, Streptavidin-HRP Concentrate, 75µL, Stop Solution, 13mL, Lyophilized Recombinant Rat IFN gamma Standard, 2 vials, Anti-Rat IFN gamma 96-well Strip Plate, 1 ea, Biotinylated Antibody Reagent, 12mL, Adhesive Plate Covers, 6 ea, Sample Diluent, 12mL, 30X Wash Buffer, 50mL
1 plate kit
Cell culture supernatant, serum, plasma (EDTA, sodium citrate and heparin)
No significant cross-reaction with rat IL-1a, IL-1ß, IL-2, IL-4, IL-6, IL-10, MIP-1a, RANTES, or TNFa, or human or pig IFNg. When preparations of NIH/NIBSC mouse IFNg (lot Gg02-901-533) were evaluated in the Rat IFNg ELISA Kit, 6250pg/mL mouse IFNg was eq
2.5 hr.
Rat IFN gamma ELISA Kit
Complete ELISA kit with coated 96-well plate
Absorbance at 450nm minus absorbance at 550nm
<2pg/mL
50µL
96 microplate wells
Natural and recombinant rat interferon gamma (IFNg)
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