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Rat anti-MHC Class II, Clone: M5/114.15.2, Novus Biologicals™

Manufacturer: novus biologicals canada  NBP14331205MG

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For Research Use Only

Catalog No. NBP1433125


Description & Specifications


Antigen MHC Class II
Applications Flow Cytometry
Applications Functional Assay
Applications Immunohistochemistry
Applications Immunohistochemistry (Frozen)
Applications Immunohistochemistry (Paraffin)
Applications Immunoprecipitation
Applications Western Blotting
Clone M5/114.15.2
Concentration 0.5mg/mL
Conjugate Unlabeled
Cross Reactivity Mouse
Format Affinity Purified
Formulation Phosphate buffer pH 7.2
Host Species Rat
Immunogen The immunogen for this antibody was MHC Class II.
Isotype IgG2b, κ
Purification Method Affinity Purified
Quantity 0.5mg
Regulatory Status RUO
Storage Requirements Store at 4°C. Do not freeze.
Primary or Secondary Primary
Monoclonal or Polyclonal Monoclonal
Gene ID (Entrez) 3108
Test Specificity The monoclonal antibody reacts with the mouse major histocompatibility complex class II, both I-A and I-E subregion-encoded glycoproteins (I-A b, I-A d, I-A q, I-E d, I-E k, not I-A f, I-A k, or I-A s). It detects a polymorphic determinant present on B cells, monocytes, macrophages, dendritic cells, and activated T lymphocytes from mice carrying the H-2 b, H-2 d, H-2 q, H-2 p, H-2 r and H-2 u but not from mice carrying the H-2 s or H-2 f haplotypes. The M5/114 mAb is reported to inhibit I-A-restricted T cell responses of the H-2 b, H-2 d, H-2 q, H-2 u but not H-2 f, H-2 k, or H-2 s haplotypes.