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RAF1 (cRAF) [Y340D] [Y341D] Recombinant Human Protein DFS Item

RAF1 (cRAF) [Y340D] [Y341D] Recombinant Human Protein

Manufacturer:  Life Technologies LSPV3805

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Catalog No. PV3805



Description

Description

RAF1 (cRAF) is a MAP kinase kinase kinase (MAP3K) which functions downstream of the Ras family of membrane associated GTPases to which it binds directly. Once activated RAF1 can phosphorylate and activate the dual specificity protein kinases MEK1 and MEK2 which in turn phosphorylates and activates the serine⁄threonine specific protein kinases ERK1 and ERK2. The RAF1 Y340D Y341D mutant is constitutively active.
Specifications

Specifications

NP_002871, Y340D, Y341D
Pharma and biopharma, protein assays and analysis, target and lead identification and validation
66.4kDa
80% by SDS-PAGE
–80°C
1 Each
RAF1 (cRAF) Y340D Y341D
RAF1 (cRAF) Y340D Y341D was pre-diluted in enzyme dilution buffer and assayed in 25mM Tris (pH 7.5), 10mM MgCl2, 0.5mM EGTA, 0.5mM Na3VO4, 5mM β-glycerophosphate, 2.5mM DTT, 0.01% Triton™ X-100, 100μM ATP, 200μg/mL MAP2K1, inactive (P3093) and trace [32P]-γ-ATP for 10 minutes at 30°C
Kinase and inhibitors
RAF
RAF1 (cRAF) [Y340D] [Y341D] Recombinant Human Protein
Human
0.31mg/mL
4773, 5894
RAF1 (cRAF) [Y340D] [Y341D] Recombinant Human Protein
10μg
For research use only. Not for use in diagnostic procedures
Serine/threonine kinase
RAF1 (cRAF) [Y340D] [Y341D] Recombinant Human Protein
Kinase assay
Baculovirus
Amino acids 306-648; Y340D, Y341D
220nmoles of phosphate transferred to MAP2K1, inactive (P3093) per minute per mg of total protein at 30°C. Activity determined at a final protein concentration of 0.25μg/mL
GST Tag
Documents

For Research Use Only. Not for use in diagnostic procedures.