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Thermo Scientific™ Proteinase K (recombinant), PCR grade


Degrade proteins even in the presence of detergents with Proteinase K, a broad-range endolytic protease widely used for digestion of proteins in nucleic acid preparations.

Manufacturer: thermo scientific™  EO0491

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Catalog No. FEREO0491

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Description & Specifications

Specifications

Concentration ~600-1,000U/mL; 14-22mg/mL
Inhibitors Proteinase K is not inactivated by metal chelators, by thiol-reactive reagents or by specific trypsin and chymotrypsin inhibitors. Phenylmethylsulfonyl fluoride and diisopropyl phosphorofluorida
Source Pichia pastoris cells with a cloned gene encoding Tritirachium album endolytic protease (Proteinase
Molecular Weight 28.9kDa monomer (6)
Quantity 1mL

Proteinase K is an endolytic protease that cleaves peptide bonds at the carboxylic sides of aliphatic, aromatic, or hydrophobic amino acids. The Proteinase K is classified as a serine protease. The smallest peptide to be hydrolyzed by this enzyme is a tetrapeptide.

Highlights

  • Ready-to-use solution
  • Active in a wide range of reaction conditions