Prostaglandin E2 Parameter Assay Kit, R&D Systems
a 3.5 hour forward sequential competitive enzyme immunoassay designed to measure PGE2 in cell culture supernates, serum, plasma, and urine
Manufacturer: R & D Systems RDSKGE004B
Kit, ELISA; R&D Systems; Prostaglandin E2 Parameter Assay Kit; One 96-well plate; 1 Kit; Sufficient materials to run ELISAs on at least one 96-well plateThis assay is based on the forward sequential competitive binding technique in which PGE2 present in a sample competes with horseradish peroxidase (HRP)-labeled PGE2 for a limited number of binding sites on a mouse monoclonal antibody. PGE2 in the sample is allowed to bind to the antibody in the first incubation. During the second incubation, HRP-labeled PGE2 binds to the remaining antibody sites. Following a wash to remove unbound materials, a substrate solution is added to the wells to determine the bound enzyme activity. The color development is stopped, and the absorbance is read at 450 nm. The intensity of the color is inversely proportional to the concentration of PGE2 in the sample.
|Cross-species reactivity not tested. Cross-reactivity observed with 1 or more available related molecules.|
|1 x 96-well plate|