Please login to your online account to display your discounted pricing

Thermo Scientific™ ProPac™ WCX-10 LC Columns


Separate proteins and their variants using these weak cation-exchange columns with exceptionally high resolution.

Manufacturer: thermo scientific™  SP5482

 View more versions of this product

 Disclaimers

Limited warranty period: Subject to all warranty requirements and exclusions set out in our Terms and Conditions of Sale, this product is warranted from the date we ship the product and for ninety (90) days thereafter. Disclaimer to internal research use restriction: Any restrictions on the purchaser to utilize the product(s) for internal research purposes only does not apply to the product(s) on the web pages associated with this statement.

Catalog No. DXSP5482

 Please call Customer Service at 1-800-234-7437 or send an email to help@thermofisher.com for assistance.


Description & Specifications

Specifications

Temperature <60�C
Flow Rate Up to 30mL/min.
Column Format Semi-Preparative Column
Diameter (Metric) 22mm
Mobile Phase Compatibility pH 2-12, 80% compatible with acetonitrile and acetone, compatible with nonionic and zwitterionic detergents, incompatible with cationic detergents
Particle Size 10µm
pH 2 to 12
Technique Protein/Cation Exchange
Carbon Load -
Resin Composition 10µm diameter core bead (55% cross-linked) grafted with a pellicular layer to which are attached the carboxylate functional groups
Endcapped -
For Use With HPLC
Max. Pressure 3000psi
Pore Size Non-porous
Stationary Phase Carboxylate
Surface Area -
Length (Metric) 250mm
Column Construction PEEK
Item Description SP5482
Type Semi-preparative
USP Type N/A
Column Type Ion Exchange
Packing Material Spherical, non-porous polymer

Use Thermo Scientific™ ProPac™ WCX-10 LC Columns to provide exceptionally high resolution for separations of proteins and their variants. Based on stationary phases composed of 10µm nonporous polymeric beads, ProPac columns resolve isoforms that differ by a single charged residue. A thin, hydrophilic layer grafted to the particle core eliminates unwanted secondary interactions. The cation-exchange surface provides pH-based selectivity control and fast mass transfer for high-efficiency separation and moderate capacity.

Multiple Formats for Greater Flexibility

  • ProPac WCX-10 column is a weak cation-exchanger with a carboxylate functional group
  • Column sizes range from 2mm I.D., for analytical work, up to 22mm I.D. for semi-prep work
Superior Performance for Protein Analysis
  • Ideal for characterization and quality control assessment of MAbs and other proteins
  • Well-suited for characterization of related protein variants including deamidation and MAb lysine truncation variants
  • Unequaled high-resolution separations
  • Unmatched column-to-column and lot-to-lot reproducibility eliminates column variability as a concern in stability and QA/QC testing