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Thermo Scientific™ ProPac™ WCX-10 LC Columns


Separate proteins and their variants using these weak cation-exchange columns with exceptionally high resolution.

Manufacturer: thermo scientific™  SP5482

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 Disclaimers

Limited warranty period: Subject to all warranty requirements and exclusions set out in our Terms and Conditions of Sale, this product is warranted from the date we ship the product and for ninety (90) days thereafter. Disclaimer to internal research use restriction: Any restrictions on the purchaser to utilize the product(s) for internal research purposes only does not apply to the product(s) on the web pages associated with this statement.

Catalog No. DXSP5482

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Description & Specifications

Specifications

Column Format Semi-Preparative Column
Flow Rate Up to 30mL/min.
Diameter (Metric) 22mm
Endcapped -
Particle Size 10µm
pH 2 to 12
Carbon Load -
Technique Protein/Cation Exchange
Mobile Phase Compatibility pH 2-12, 80% compatible with acetonitrile and acetone, compatible with nonionic and zwitterionic detergents, incompatible with cationic detergents
USP Type N/A
Length (Metric) 250mm
Max. Pressure 3000psi
Stationary Phase Carboxylate
Temperature 60°C
Pore Size Non-porous
Surface Area -
Packing Material Spherical, non-porous polymer
Column Type Ion Exchange

Use Thermo Scientific™ ProPac™ WCX-10 LC Columns to provide exceptionally high resolution for separations of proteins and their variants. Based on stationary phases composed of 10µm nonporous polymeric beads, ProPac columns resolve isoforms that differ by a single charged residue. A thin, hydrophilic layer grafted to the particle core eliminates unwanted secondary interactions. The cation-exchange surface provides pH-based selectivity control and fast mass transfer for high-efficiency separation and moderate capacity.

Multiple Formats for Greater Flexibility

  • ProPac WCX-10 column is a weak cation-exchanger with a carboxylate functional group
  • Column sizes range from 2mm I.D., for analytical work, up to 22mm I.D. for semi-prep work
Superior Performance for Protein Analysis
  • Ideal for characterization and quality control assessment of MAbs and other proteins
  • Well-suited for characterization of related protein variants including deamidation and MAb lysine truncation variants
  • Unequaled high-resolution separations
  • Unmatched column-to-column and lot-to-lot reproducibility eliminates column variability as a concern in stability and QA/QC testing