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Thermo Scientific™ ProPac™ SAX-10 LC Columns


Use ProPac™ SAX-10 LC Columns for exceptionally high resolution and efficiency when separating protein variants.

Manufacturer: thermo scientific™  SP5730

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 Disclaimers

Limited warranty period: Subject to all warranty requirements and exclusions set out in our Terms and Conditions of Sale, this product is warranted from the date we ship the product and for ninety (90) days thereafter. Disclaimer to internal research use restriction: Any restrictions on the purchaser to utilize the product(s) for internal research purposes only does not apply to the product(s) on the web pages associated with this statement.

Catalog No. DXSP5730

  • / Pack of 3 Each
 

Description & Specifications

Specifications

Column Capacity <100μg
Temperature <60�C
Mobile Phase Compatibility pH 2-12, 80% Compatible with Acetonitrile, Acetone, Methanol, Compatible with Nonionic and Zwitterionic Detergents, Incompatible with Anionic Detergents
Packing Material Spherical, Non-Porous Polymer
Pore Size Non-Porous
Stationary Phase Quaternary Ammonium
Surface Area
Carbon Load
Column Format 3 Lots of Resin, 1 Column Per Lot (3 Columns)
Endcapped
Diameter (Metric) 4mm
Column Type Ion Exchange
Flow Rate 1mL/min.
Max. Pressure 3000psi
Particle Size 10µm
For Use With UV-Vis Detector
Technique Protein/Anion Exchange
Item Description 3 lots of resin, 1 column from each lot
Resin Composition 10µm diameter core bead (55% cross-linked) grafted with a pellicular layer to which are attached the quaternary ammonium functional groups
Type 3 lots of resin, 1 column from each lot
pH 2 to 12
Column Construction PEEK
Length (Metric) 250mm
USP Type N/A

Use Thermo Scientific™ ProPac™ SAX-10 LC Columns for exceptionally high resolution and efficiency when separating protein variants. Based on a nonporous 10µm core particle that provides pressure stability, ProPac columns can resolve isoforms that differ by a single charged residue. A thin, hydrophilic layer prevents unwanted secondary interactions. The grafted anion-exchange surface provides pH-based selectivity control and fast mass transfer for high-efficiency separation and moderate capacity.

Maximizing Resolution

  • ProPac SAX-10 LC column is a strong anion-exchanger with a quaternary amine functional group.
  • Column sizes range from 2mm I.D., for analytical work, up to 22mm I.D. for semiprep work.

Superior Performance for Protein Analysis
  • Ideal for characterization and quality control assessment of closely-related protein variants
  • Unequaled high-resolution separations
  • High-efficiency peaks with excellent recovery
  • Higher capacity than other columns based on nonporous particles
  • Superior column-to-column and lot-to-lot reproducibility eliminates column variability as a concern in stability and QA/QC testing