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Thermo Scientific™ ProPac™ SAX-10 LC Columns

Use ProPac™ SAX-10 LC Columns for exceptionally high resolution and efficiency when separating protein variants.

Manufacturer: thermo scientific™  SP5730

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Catalog No. DXSP5730

  • / Pack of 3 Each

Description & Specifications


Column Type Ion Exchange
For Use With UV-Vis Detector
Carbon Load
USP Type N/A
Flow Rate 1mL/min.
Max. Pressure 3000psi
Mobile Phase Compatibility pH 2-12, 80% Compatible with Acetonitrile, Acetone, Methanol, Compatible with Nonionic and Zwitterionic Detergents, Incompatible with Anionic Detergents
Packing Material Spherical, Non-Porous Polymer
Particle Size 10µm
Temperature 60°C
Pore Size Non-Porous
pH 2 to 12
Stationary Phase Quaternary Ammonium
Column Capacity <100�g
Surface Area
Length (Metric) 250mm
Technique Protein/Anion Exchange
Column Format 3 Lots of Resin, 1 Column Per Lot (3 Columns)
Diameter (Metric) 4mm

Use Thermo Scientific™ ProPac™ SAX-10 LC Columns for exceptionally high resolution and efficiency when separating protein variants. Based on a nonporous 10µm core particle that provides pressure stability, ProPac columns can resolve isoforms that differ by a single charged residue. A thin, hydrophilic layer prevents unwanted secondary interactions. The grafted anion-exchange surface provides pH-based selectivity control and fast mass transfer for high-efficiency separation and moderate capacity.

Maximizing Resolution

  • ProPac SAX-10 LC column is a strong anion-exchanger with a quaternary amine functional group.
  • Column sizes range from 2mm I.D., for analytical work, up to 22mm I.D. for semiprep work.

Superior Performance for Protein Analysis
  • Ideal for characterization and quality control assessment of closely-related protein variants
  • Unequaled high-resolution separations
  • High-efficiency peaks with excellent recovery
  • Higher capacity than other columns based on nonporous particles
  • Superior column-to-column and lot-to-lot reproducibility eliminates column variability as a concern in stability and QA/QC testing