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Thermo Scientific™ ProPac™ SAX-10 LC Columns


Use ProPac™ SAX-10 LC Columns for exceptionally high resolution and efficiency when separating protein variants.

Manufacturer: thermo scientific™  SP5594

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 Disclaimers

Limited warranty period: Subject to all warranty requirements and exclusions set out in our Terms and Conditions of Sale, this product is warranted from the date we ship the product and for ninety (90) days thereafter. Disclaimer to internal research use restriction: Any restrictions on the purchaser to utilize the product(s) for internal research purposes only does not apply to the product(s) on the web pages associated with this statement.

Catalog No. DXSP5594

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Description & Specifications

Specifications

For Use With LC
Endcapped
Packing Material Spherical, Non-Porous Polymer
Stationary Phase Quaternary Ammonium
Surface Area
Carbon Load
Mobile Phase Compatibility pH 2-12, 80% Compatible with Acetonitrile, Acetone, Methanol, Compatible with Nonionic and Zwitterionic Detergents, Incompatible with Anionic Detergents
Pore Size Non-Porous
Column Format Analytical Column
Diameter (Metric) 22mm
Flow Rate 1mL/min.
USP Type N/A
Length (Metric) 250mm
Column Type Ion Exchange
Technique Protein/Anion Exchange
Temperature 60°C
Max. Pressure 3000psi
Particle Size 10µm
pH 2 to 12

Use Thermo Scientific™ ProPac™ SAX-10 LC Columns for exceptionally high resolution and efficiency when separating protein variants. Based on a nonporous 10µm core particle that provides pressure stability, ProPac columns can resolve isoforms that differ by a single charged residue. A thin, hydrophilic layer prevents unwanted secondary interactions. The grafted anion-exchange surface provides pH-based selectivity control and fast mass transfer for high-efficiency separation and moderate capacity.

Maximizing Resolution

  • ProPac SAX-10 LC column is a strong anion-exchanger with a quaternary amine functional group.
  • Column sizes range from 2mm I.D., for analytical work, up to 22mm I.D. for semiprep work.

Superior Performance for Protein Analysis
  • Ideal for characterization and quality control assessment of closely-related protein variants
  • Unequaled high-resolution separations
  • High-efficiency peaks with excellent recovery
  • Higher capacity than other columns based on nonporous particles
  • Superior column-to-column and lot-to-lot reproducibility eliminates column variability as a concern in stability and QA/QC testing