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Thermo Scientific™ ProPac™ IMAC-10 LC Columns


Resolve target proteins using a single column in a high-resolution gradient run with ProPac IMAC-10 LC Columns.

Manufacturer: thermo scientific™  063718

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Catalog No. DX063718

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Description & Specifications

Specifications

Column Type Affinity
For Use With ProPac IMAC-10 Column
Type Loading Column
Carbon Load -
USP Type N/A
Endcapped -
Flow Rate 0.1 to 10mL/min.
Max. Pressure 3000psi
Packing Material Spherical, non-porous polymer
Particle Size 10µm
Temperature 60°C
Pore Size Non-porous
pH 2 to 12
Stationary Phase Poly (IDA) grafts
Surface Area -
Length (Metric) 250mm
Column Format Loading Column
Diameter (Metric) 9mm

Resolve target proteins using a single column in a high-resolution gradient run with Thermo Scientific™ ProPac™ IMAC-10 LC Columns. These analytical and semi-preparative HPLC columns provides high-purity separations of metal-binding proteins and high loading capacity for protein purification applications. The use of small, nonporous resin particles results in narrow, high-efficiency peaks, with minimal band broadening due to dispersion. Use loading columns to charge the ProPac IMAC-10 LC columns but avoid contact of the metal solution with the system.

Tailored Specificity for IMAC Separation of Proteins

  • Retention control by imidazole concentration or pH gradient
  • Stable between pH 2 to 12, compatible with common protein purification reagents (nonionic detergents, reducing agents)
  • Effective under native or denaturing HPLC conditions
  • Wide range of metal-specific applications, including the separation of His-tagged protein aggregation variants, prion peptide analysis and separation of monoclonal antibodies
  • Shipped free from any metal and ready to be charged with the metal of your choice.
  • Reusable columns with metal tailored specificity
  • Resolve target proteins using a single column in a high resolution gradient run
  • High purity separations of metal-binding proteins
Includes: 10µm, nonporous, polymeric beads coated with a hydrophilic layer, then grafted with poly(IDA) chains.