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Promega™ Wizard™ SV Gel and PCR Cleanup System


Extract DNA fragments from standard or low-melt agarose gels or purify PCR products directly from an amplification reaction. Purified DNA can be used for automated fluorescent DNA sequencing, cloning, labeling, restriction enzyme digestion, or in vitro transcription/translation.

Manufacturer: promega™  A9282

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Vacuum protocol requires vacuum adapters (A1331, sold separately).

Catalog No. PR-A9282

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Description & Specifications

Specifications

For Use With (Application) Purifies DNA from agarose gels or PCR reactions in as little as 15 min.
Format Spin or Vacuum
Includes 100mL Membrane binding solution, 75mL membrane wash solution, 13mL Nuclease-free Water, 250 Wizard™ SV minicolumns, 250 2mL collection tubes
Product Type System
Quantity 250 Preps

Membrane-based system purifies DNA fragments from standard or low-melt agarose gels in either Tris acetate (TAE) or Tris borate (TBE). Also purifies products directly from PCR reactions, removing excess nucleotides, and primers.

  • Effectively extracts DNA ranging from 100bp to 10kb
  • Recovers up to 95% of fragments or amplification products in as little as 15μL elution volume
  • Suitable for spin or vacuum purification formats; no phenol extraction or ethanol precipitation required
  • Tested to ensure performance of purified products in automated fluorescent sequencing to at least 700 bp with 78% accuracy