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Promega VivoGlo™ In Vivo Imaging Substrates DFS Item

VivoGlo™ Luciferin is the potassium salt of D-luciferin, the firefly luciferase substrate capable of generating light when a suitable model is used.

Manufacturer:  Promega P1043

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Catalog No. PRP1043



  • Eliminates potential interference in assays due to the presence of endotoxins
  • Most products packaged in amber vials with septa to ensure product integrity as well as offer ease of dilution and use for imaging experiments
  • Product is packaged with fine tolerances to minimize the need to weigh substrates
  • Available in multiple sizes to accommodate a variety of experimental settings

VivoGlo Luciferin, in vivo grade

  • Potassium salt of D-luciferin
  • VivoGlo Caspase-3/7 Substrate (Z-DEVD-Aminoluciferin)
    • Firefly luciferase substrate protected by the caspase-3 and -7 peptide substrate
    • Upon activation of caspase-3 or -7, the DEVD-peptide is cleaved to release aminoluciferin
    • When used in a suitable model animal system containing firefly luciferase, the liberated aminoluciferin will react with luciferase to generate measurable light
    VivoGlo Luciferin-β-Galactoside Substrate (6-O-β-galactopyranosyl-luciferin)
    • Luciferin-β-galactoside is a substrate for the commonly used reporter enzyme β-galactosidase
    • Cleaved by β-galactosidase to form luciferin and galactose
    • When used in a model system expressing firefly luciferase, the luciferin is then utilized in a firefly luciferase reaction to generate light



    In-vivo bioluminescence imaging applications
    VivoGlo Luciferin
    −30 to −10°C