Promega™ VivoGlo™ Caspase-3/7 Substrate (Z-DEVD-Aminoluciferin, Sodium Salt)
VivoGlo™ Caspase-3/7 Substrate (Z-DEVD-Aminoluciferin, Sodium Salt) is a firefly luciferase prosubstrate containing the DEVD tetrapeptide sequence recognized by caspase-3 and -7.
Manufacturer: Promega™ P1781
- Upon activation of caspase-3 or -7, the DEVD peptide is cleaved, and the liberated aminoluciferin reacts with luciferase to generate measurable light
- Cleavage has been shown in in cellulo and in vivo systems
- The substrate has a minimum solubility of 500mg/mL in PBS, and the resulting solution is stable for at least three days at room temperature
- Injection is usually done via the intraperitoneal route, and imaging is generally started ten minutes after injection
|Eliminate potential interference in assays due to the presence of endotoxins, in-vivo bioluminescence imaging applications|
|−30 to −10°C|
The use of this product and derivatives thereof is strictly limited to that of a life sciences research reagent. All other use is strictly prohibited, including but not limited to any diagnostic, therapeutic, or commercial use. The use of this product for in vivo bioluminescent imaging may be covered by one or more patents controlled by Caliper Life Sciences, Inc. Those patents include U.S. Patents 5,650,135; 6,217,847; 6,923,951; 6,908,605; 6,890,515; and corresponding foreign patents and pending applications. The purchase or transfer of this product is not intended, either expressly or by implication, to grant any right or license to practice under the foregoing patents. Purchasers are advised to contact Caliper Life Sciences, Inc., 68 Elm St., Hopkinton, MA 01748 for information regarding a license to practice under such patents.
This product does not convey a license to use recombinant Coleoptera luciferase under U.S. Patents 5,583,024; 5,674,713 and 5,700,673. Promega sells licensed Coleoptera luciferase vectors, which may be used in conjunction with this product.