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Promega™ Viral ToxGlo™ Assay Systems DFS Item

The Viral ToxGlo™ Assay is a simple, quantifiable method of determining viral-induced cytopathic effects (CPE) in host cells caused by lytic virions. The assay measures cellular ATP as a surrogate measure of host cell viability.

Manufacturer:  Promega™ G8943

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Catalog No. PRG8943


Alert:

This method is only useful for viruses that produce cytotoxicity and CPE. Not for use in diagnostic procedures.


Description

Description

The assay measures cellular ATP as a surrogate measure of host cell viability. When CPE occurs because of viral infection, ATP depletion can be measured and correlated with viral burden. The amount of ATP detected is directly proportional to the number of viable host cells in culture and can be used as a simple method to quantify viral-induced CPE.

  • Provide quantifiable data by luminescence detection, which obviates subjective operator error associated with visual scoring methods
  • Data can be recorded and analysis begun 10 minutes after reagent addition
  • Homogeneous “add-mix-measure” protocol reduces manual steps required for CPE assessment
  • “Glow-type” luminescent signal is generated that is proportional to the amount of ATP present
  • Cell washing, multiple pipetting steps and visual assessment are not required to assess CPE
  • System detects as few as 15 cells/well in a 384-well format in 10 minutes after reagent addition and mixing
  • Reagent is scalable from 96- to 1536-well plate formats (multiwell formats), making it ideal for automated high-throughput screening (HTS)
  • Luminescent signal is very stable with half-life generally >5 hours dependent on cell type and medium used, allowing batch or consecutive processing
  • No fluorescence interference results in high signal to background and delivers excellent Z values in screening applications

  • Determine viral infectivity and corresponding tissue culture infective dose
  • Determine potential antiviral potency or off-target toxicity of test compounds

Specifications

Specifications

1 x 100mL
Luminescence
Determining viral-induced cytopathic effects (CPE) in host cells caused by lytic virions
-30°C to -10°C.
ATP detection buffer and ATP detection substrate
Assay Systems
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Research use only.