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Promega™ Transcend™ Nonradioactive Translation Detection Systems


Allow nonradioactive detection of proteins synthesized in vitro

Manufacturer: promega™  L5080

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Catalog No. PR-L5080

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Description & Specifications

Specifications

For Use With (Application) Non-radioactive detection of Proteins synthesized in-vitro
Includes 30μL Transcend™ tRna, 0.25mg/mL 15μL Streptavidin-HRP, 25mL Transcend™ Chemiluminescent substrate A and B
Product Type Transcend Chemiluminescent Nonradioactive Translation Detection System
Quantity 30 x 50μL Reactions

Using these systems, biotinylated lysine residues are incorporated into nascent proteins during translation, eliminating the need for labeling with [35S]methionine or other radioactive amino acids. This biotinylated lysine is added to the translation reaction as a precharged ε-labeled biotinylated lysine-tRNA complex (Transcend tRNA) rather than a free amino acid. After SDS-PAGE and electroblotting, the biotinylated proteins can be visualized by binding either Streptavidin-Alkaline Phosphatase (Streptavidin-AP) or Streptavidin-Horseradish Peroxidase (Streptavidin-HRP), followed either by colorimetric or chemiluminescent detection.

  • Sensitive: The biotin tag allows detection of 0.5 to 5ng of translated protein
  • Safe: No radioisotope handling, storage or disposal is required
  • Fast: Labeled proteins can be detected three to four hours after gel electrophoresis
  • Flexible: Results can be visualized by using colorimetric or chemiluminescent detection
  • IRNA available separately (PR-L5061)