Promega™ TNT™ T7 Insect Cell Extract Protein Expression System
The TNT T7 Insect Cell Extract Protein Expression System is a convenient, quick, single-tube, coupled transcription/translation system for cell-free protein expression.
Manufacturer: Promega™ L1102
- System utilizes extract that is made from the commonly used Spodoptera frugiperda Sf21 cell line
- Master mix contains all components necessary for transcription/translation, except DNA template
- pF25A or pF25K ICE T7 Flexi™ Expression Vectors1, 2, 3, 4 (available separately, Mfr. Nos. L1061 and L1081, respectively) are strongly recommended for maximal protein expression with the TNT T7 Insect Cell Extract Protein Expression System2, 5, 6
- Yields of up to 75μg/mL of functional protein can be produced when the system is used with pF25A or pF25K vectors
- Reactions are complete within four hours, not days as with cell-based expression
|TnT™ T7 ICE master mix, luciferase ICE T7 control DNA, Nuclease-free Water|
|TnT™ T7 Insect cell extract Protein Expression system|
Purchase up to $1,000 in Promega™ reagents and get a matching amount of products at no additional cost.
Purchase up to $1,000 in Promega reagents and get a matching amount of products at no additional cost.