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Promega™ TNT™ T7 Insect Cell Extract Protein Expression System DFS Item

The TNT T7 Insect Cell Extract Protein Expression System is a convenient, quick, single-tube, coupled transcription/translation system for cell-free protein expression.

Manufacturer:  Promega™ L1101

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Catalog No. PRL1101

Includes: TNT T7 ICE Master Mix, Luciferase ICE T7 Control DNA, Nuclease-free water



  • System utilizes extract that is made from the commonly used Spodoptera frugiperda Sf21 cell line
  • Master mix contains all components necessary for transcription/translation, except DNA template
  • pF25A or pF25K ICE T7 Flexi™ Expression Vectors1, 2, 3, 4 (available separately, Mfr. Nos. L1061 and L1081, respectively) are strongly recommended for maximal protein expression with the TNT T7 Insect Cell Extract Protein Expression System2, 5, 6
  • Yields of up to 75μg/mL of functional protein can be produced when the system is used with pF25A or pF25K vectors
  • Reactions are complete within four hours, not days as with cell-based expression



TnT™ T7 ICE master mix, luciferase ICE T7 control DNA, Nuclease-free Water
10 Reactions
4 hr.
TnT™ T7 Insect cell extract Protein Expression system