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Promega™ TNT™ SP6 High-Yield Protein Expression System DFS Item

TNT SP6 High-Yield Wheat Germ Protein Expression System, based on an optimized wheat germ extract, is a single-tube, coupled transcription/translation system designed to express proteins in only two hours.

Manufacturer:  Promega™ L3260*

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Catalog No. PRL3260

Includes: TNT SP6 High-Yield Master Mix, Nuclease-free water



  • Based on an optimized wheat germ extract
  • Contains all components necessary for transcription/translation, with the exception of the plasmid DNA or PCR template and optional protein-labeling reagents
  • Yields 10 to 100μg/mL protein, 10- to 20-fold more protein compared to other cell-free systems
  • Generates soluble, full-length protein and avoids problems associated with Escherichia coli systems
  • Produces protein that can be used in multiple proteomic-based applications, as well as in high-throughput analysis



TnT™ SP6 high-yield master mix, Nuclease-free Water
40 Reactions
2 hr.
TnT™ SP6 high yield Wheat Germ Protein Expression system
10 to 100μg/mL


U.S. Patent 5,324,637; Australian Patent 660329 and other patents.
For Research Use. Any use of the product for diagnostics requiring clearance or approval by the FDA may require a license under Mayo Clinic U.S. Patents 6,027,913 and 6,361,949.