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Promega™ TNT™ Quick Coupled Transcription/Translation System


Convenient cell-free system combines RNA polymerase, nucleotides, salts, amino acids, and Recombinant RNasin™ Ribonuclease Inhibitor with reticulocyte lysate solution in a single TNT Quick Master Mix

Manufacturer: promega™  L1171

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Catalog No. PR-L1171

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Description & Specifications

Specifications

For Use With (Application) GST pull-downs, Gel shift assays, Co-immunoprecipitation, Characterization of protein modifications, Protein-RNA interactions, Protein activity studies, Confirmation of gene products, Protein truncation test (PTT)
Includes 200μL TNT T7 Quick Master Mix, 5μg Luciferase T7 Control DNA, 100μL T7 TNT PCR Enhancer, 50μL Methionine
Product Type TNT T7 Quick Coupled Transcription/Translation System
Quantity 5 Reactions
Storage Requirements Store at -70°C; do not freeze-thaw the lysate more than two times.

  • Optimized for transcription/translation of genes cloned downstream from either the T7 or SP6 RNA polymerase promoter
  • Produces two- to six-fold more protein in a 60- to 90-minute reaction than do standard in vitro rabbit reticulocyte lysate reactions using RNA templates
  • Includes Luciferase Control DNA; 40-reaction systems also include Luciferase Assay Reagent