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Promega™ TNT™ Quick Coupled Transcription/Translation System

Convenient cell-free system combines RNA polymerase, nucleotides, salts, amino acids, and Recombinant RNasin™ Ribonuclease Inhibitor with reticulocyte lysate solution in a single TNT Quick Master Mix

Manufacturer: promega™  L1170

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Catalog No. PR-L1170

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Description & Specifications


For Use With (Application) GST pull-downs, Gel shift assays, Co-immunoprecipitation, Characterization of protein modifications, Protein-RNA interactions, Protein activity studies, Confirmation of gene products, Protein truncation test (PTT)
Includes 8 × 200μL TNT Quick Master Mix, 5μg Luciferase T7 Control DNA, 100μL T7 TNT PCR Enhancer, 250μL Luciferase Assay Reagent, 50μL Methionine, 1250μL Nuclease-free Water
Product Type TNT T7 Quick Coupled Transcription/Translation System
Quantity 40 Reactions
Storage Requirements Store at -70°C; do not freeze-thaw the lysate more than two times.

  • Optimized for transcription/translation of genes cloned downstream from either the T7 or SP6 RNA polymerase promoter
  • Produces two- to six-fold more protein in a 60- to 90-minute reaction than do standard in vitro rabbit reticulocyte lysate reactions using RNA templates
  • Includes Luciferase Control DNA; 40-reaction systems also include Luciferase Assay Reagent