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Promega™ TNT™ Quick Coupled Transcription/Translation System

Convenient cell-free system combines RNA polymerase, nucleotides, salts, amino acids, and Recombinant RNasin™ Ribonuclease Inhibitor with reticulocyte lysate solution in a single TNT Quick Master Mix

Manufacturer: promega™  L2081

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Catalog No. PR-L2081


Description & Specifications


For Use With (Application) GST pull-downs, Gel shift assays, Co-immunoprecipitation, Characterization of protein modifications, Protein-RNA interactions, Protein activity studies, Confirmation of gene products, Protein truncation test (PTT)
Includes 200μL TNT SP6 Quick Master Mix, 5μg Luciferase SP6 Control DNA, 50μL Methionine
Product Type TNT SP6 Quick Coupled Transcription/Translation System
Quantity 5 Reactions
Storage Requirements Store at -70°C; do not freeze-thaw the lysate more than two times.

  • Optimized for transcription/translation of genes cloned downstream from either the T7 or SP6 RNA polymerase promoter
  • Produces two- to six-fold more protein in a 60- to 90-minute reaction than do standard in vitro rabbit reticulocyte lysate reactions using RNA templates
  • Includes Luciferase Control DNA; 40-reaction systems also include Luciferase Assay Reagent