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Promega TNT™ Coupled Reticulocyte Lysate Systems
TNT Coupled Reticulocyte Lysate Systems are single-tube, coupled transcription/translation systems for eukaryotic cell-free protein expression.
Supplier: Promega L4611
Includes: TNT Rabbit Reticulocyte Lysate, Appropriate TNT polymerase, Control DNA, TNT Reaction Buffer, Luciferase assay wells, Luciferase assay reagent, Amino acid mixtures
Description
- A Single-Tube, Coupled Transcription/Translation System
- Study protein:protein, protein:nucleic acid and protein expression interactions
- Generate proteins in one hour versus days
- You choose the promoter
U.S. Patents 5,324,637 and 5,492,817; Australian Patent 660329.
U.S. Patents 5,641,641; 5,650,289; and 5,814,471; Australian Patent 649289.
Australian Patents 616881 and 641261 and other pending and issued patents, which claim vectors encoding a portion of human placental ribonuclease inhibitor, are exclusively licensed to Promega Corporation.
The method of recombinant expression of Coleoptera luciferase is covered by U.S. Patents 5,583,024; 5,674,713; and 5,700,673. A license (from Promega for research reagent products and from The Regents of the University of California for all other fields) is needed for any commercial sale of nucleic acid contained within or derived from this product.
Specifications
<-65°C | |
200μL TNT™ Rabbit Reticulocyte Lysate, 20μL TNT™ Reaction Buffer, 20μL TNT™ T7 or SP6 RNA Polymerase, 5μg Luciferase T7 or SP6 co | |
TnT T7 coupled reticulocyte lysate system |
GST pull-downs, Gel shift assays, co-immunoprecipitation, characterization of Protein modifications, Protein-RNA interactions, Protein activity studies, confirmation of gene products, Protein truncation test (PTT) | |
8 Reactions - Trial Size |