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Promega™ TNT™ Coupled Reticulocyte Lysate Systems

Single-tube, coupled transcription/translation systems for eukaryotic in vitro translations

Manufacturer: promega™  L4601

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Catalog No. PR-L4601


Description & Specifications


For Use With (Application) GST pull-downs, Gel shift assays, co-immunoprecipitation, characterization of Protein modifications, Protein-RNA interactions, Protein activity studies, confirmation of gene products, Protein truncation test (PTT)
Includes 200μL TNT™ Rabbit Reticulocyte Lysate, 20μL TNT™ Reaction Buffer, 20μL TNT™ T7 or SP6 RNA Polymerase, 5μg Luciferase T7 or SP6 co
Product Type TnT SP6 coupled reticulocyte lysate system
Quantity 8 Reactions - Trial Size

  • Produce data in as little as five to six hours, half the time of conventional two-step systems
  • Provide two to six times the protein of standard translation reactions
  • Compatible with linear or circular templates (T7 and T3 systems only; SP6 systems should be used with circular DNA only)
  • Rigorously quality controlled to ensure the highest level of transcription/translation
  • Available in configurations that permit co-expression of genes under the control of different phage polymerase promoters