Promega™ Terminal Deoxynucleotidyl Transferase
TdT catalyzes the addition of mononucleotides to the terminal 3′-OH of a DNA initiator accompanied by the release of inorganic phosphate.
Manufacturer: Promega™ M1871
|Terminal Deoxynucleotidyl Transferase|
|5X Reaction Buffer: 500mM Cacodylate Buffer (pH 6.8 at 25°C), 5mM CoCl2, 0.5mM DTT|
|Tailing reactions to add complementary Homopolymer tails to DNA vectors and cDNA, 3' end-labeling, TUNEL Assays|
|−30 to −10°C|
|Recombinant E. coli strain|
|50mM Potassium Phosphate (pH 6.4), 100mM NaCl, 1mM beta-mercaptoethanol, 0.1% Tween™ 20, 50% Glycerol|
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