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Promega™ T4 Polynucleotide Kinase DFS Item

Catalyzes the transfer of the γ-phosphate from ATP to the 5´-terminus of polynucleotides or to mononucleotides bearing a 5´-hydroxyl group. Promega T4 Polynucleotide Kinase Enzyme, purified from recombinant E. coli, may be used to phosphorylate RNA, DNA and synthetic oligonucleotides prior to subsequent manipulations such as ligation.

Manufacturer:  Promega™ M4103

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Catalog No. PR-M4103


 

Specifications Description & Specifications

Specifications

10X Reaction Buffer: 700mM Tris HCl (pH 7.6 at 25°C), 100mM MgCl2, 50mM DTT
5′ End-labeling of single or double-stranded DNA and RNA molecules for use as probes, sequencing or DNA Protein footprinting, Phosphorylation of DNA prior to cloning
-20°C
Colorless
≥90%
Liquid
T4 Polynucleotide Kinase
Recombinant E. coli strain
20mM Tris HCl (pH 7.5), 25mM KCl, 2mM DTT, 0.1mM EDTA, 0.1μM ATP, 50% Glycerol
7.5
Ammonium Ions and low levels of Phosphate Buffers
1000U
132
5 to 10U/μL