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Promega™ T4 Polynucleotide Kinase DFS Item

Catalyzes the transfer of the γ-phosphate from ATP to the 5´-terminus of polynucleotides or to mononucleotides bearing a 5´-hydroxyl group. Promega T4 Polynucleotide Kinase Enzyme, purified from recombinant E. coli, may be used to phosphorylate RNA, DNA and synthetic oligonucleotides prior to subsequent manipulations such as ligation.

Manufacturer:  Promega™ M4103

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Catalog No. PR-M4103


Specifications Description & Specifications


10X Reaction Buffer: 700mM Tris HCl (pH 7.6 at 25°C), 100mM MgCl2, 50mM DTT
5′ End-labeling of single or double-stranded DNA and RNA molecules for use as probes, sequencing or DNA Protein footprinting, Phosphorylation of DNA prior to cloning
T4 Polynucleotide Kinase
Recombinant E. coli strain
20mM Tris HCl (pH 7.5), 25mM KCl, 2mM DTT, 0.1mM EDTA, 0.1μM ATP, 50% Glycerol
Ammonium Ions and low levels of Phosphate Buffers
5 to 10U/μL