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Promega™ T4 Polynucleotide Kinase DFS Item


Catalyzes the transfer of the γ-phosphate from ATP to the 5´-terminus of polynucleotides or to mononucleotides bearing a 5´-hydroxyl group. Promega T4 Polynucleotide Kinase Enzyme, purified from recombinant E. coli, may be used to phosphorylate RNA, DNA and synthetic oligonucleotides prior to subsequent manipulations such as ligation.

Manufacturer: promega™  M4103

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Catalog No. PR-M4103

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Description & Specifications

Specifications

Includes 10X Reaction Buffer: 700mM Tris HCl (pH 7.6 at 25°C), 100mM MgCl2, 50mM DTT
Source Recombinant E. coli strain
For Use With (Application) 5′ End-labeling of single or double-stranded DNA and RNA molecules for use as probes, sequencing or DNA Protein footprinting, Phosphorylation of DNA prior to cloning
Storage Buffer 20mM Tris HCl (pH 7.5), 25mM KCl, 2mM DTT, 0.1mM EDTA, 0.1μM ATP, 50% Glycerol
Storage Requirements -20°C
pH 7.5
Color Colorless
Inhibitors Ammonium Ions and low levels of Phosphate Buffers
Purity ≥90%
Quantity 1000U
Format Liquid
Molecular Weight 132
Product Name T4 Polynucleotide Kinase
Concentration 5 to 10U/μL