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Promega™ T4 Polynucleotide Kinase DFS Item

Catalyzes the transfer of the γ-phosphate from ATP to the 5´-terminus of polynucleotides or to mononucleotides bearing a 5´-hydroxyl group. Promega T4 Polynucleotide Kinase Enzyme, purified from recombinant E. coli, may be used to phosphorylate RNA, DNA and synthetic oligonucleotides prior to subsequent manipulations such as ligation.

Manufacturer:  Promega™ M4101

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Catalog No. PR-M4101


 

Specifications

Description

Enzyme, Modifying, T4 Polynucleotide Kinase; Promega; 100U; Conc.: 5-10U/uL; Format: Liquid

  • May Be Heat-Inactivated: T4 Polynucleotide Kinase may be inactivated by heating at 68°C for 10 minutes
  • Provided with 10X Reaction Buffer: 700mM Tris-HCl (pH 7.6 at 25°C), 100mM MgCl2, 50mM DTT
  • Blue/White Cloning Qualified: Promega's blue/white cloning assay provides higher level of quality control for enzymes used in cloning applications
Applications:
  • 5´ end-labeling of single- or double-stranded DNA and RNA molecules for use as probes, for sequencing or for DNA-protein footprinting
  • Phosphorylation of DNA prior to cloning
Description & Specifications

Specifications

100U
Recombinant E. coli strain
5′ End-labeling of single or double-stranded DNA and RNA molecules for use as probes, sequencing or DNA Protein footprinting, Phosphorylation of DNA prior to cloning
-20°C
Colorless
5 to 10U/μL
132
T4 Polynucleotide Kinase
7.5
Liquid
≥90%
10X Reaction Buffer: 700mM Tris HCl (pH 7.6 at 25°C), 100mM MgCl2, 50mM DTT
Ammonium Ions and low levels of Phosphate Buffers
20mM Tris HCl (pH 7.5), 25mM KCl, 2mM DTT, 0.1mM EDTA, 0.1μM ATP, 50% Glycerol