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Promega™ T4 Polynucleotide Kinase


Catalyzes the transfer of the γ-phosphate from ATP to the 5´-terminus of polynucleotides or to mononucleotides bearing a 5´-hydroxyl group. Promega T4 Polynucleotide Kinase Enzyme, purified from recombinant E. coli, may be used to phosphorylate RNA, DNA and synthetic oligonucleotides prior to subsequent manipulations such as ligation.

Manufacturer: promega™  M4101

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Catalog No. PR-M4101

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Description & Specifications

Specifications

Product Name T4 Polynucleotide Kinase
Format Liquid
Concentration 5 to 10U/μL
Color Colorless
Inhibitors Ammonium Ions and low levels of Phosphate Buffers
Source Recombinant E. coli strain
Purity ≥90%
pH 7.5
Molecular Weight 132
Includes 10X Reaction Buffer: 700mM Tris HCl (pH 7.6 at 25°C), 100mM MgCl2, 50mM DTT
Quantity 100U
For Use With (Application) 5′ End-labeling of single or double-stranded DNA and RNA molecules for use as probes, sequencing or DNA Protein footprinting, Phosphorylation of DNA prior to cloning
Storage Buffer 20mM Tris HCl (pH 7.5), 25mM KCl, 2mM DTT, 0.1mM EDTA, 0.1μM ATP, 50% Glycerol
Storage Requirements -20°C

  • May Be Heat-Inactivated: T4 Polynucleotide Kinase may be inactivated by heating at 68°C for 10 minutes
  • Provided with 10X Reaction Buffer: 700mM Tris-HCl (pH 7.6 at 25°C), 100mM MgCl2, 50mM DTT
  • Blue/White Cloning Qualified: Promega's blue/white cloning assay provides higher level of quality control for enzymes used in cloning applications
Applications:
  • 5´ end-labeling of single- or double-stranded DNA and RNA molecules for use as probes, for sequencing or for DNA-protein footprinting
  • Phosphorylation of DNA prior to cloning