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Promega™ T4 Polynucleotide Kinase DFS Item

T4 PNK catalyzes the transfer of the gamma-phosphate from ATP to the 5′-terminus of polynucleotides or to mononucleotides bearing a 5′-hydroxyl group.

Manufacturer:  Promega™ M4101

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Catalog No. PR-M4101



Description

Description

  • May Be Heat-Inactivated: T4 Polynucleotide Kinase may be inactivated by heating at 68°C for 10 minutes
  • Provided with 10X Reaction Buffer: 700mM Tris-HCl (pH 7.6 at 25°C), 100mM MgCl2, 50mM DTT
  • Blue/White Cloning Qualified: Promega's blue/white cloning assay provides higher level of quality control for enzymes used in cloning applications
Applications:
  • 5´ end-labeling of single- or double-stranded DNA and RNA molecules for use as probes, for sequencing or for DNA-protein footprinting
  • Phosphorylation of DNA prior to cloning
Specifications

Specifications

T4 Polynucleotide Kinase
5 to 10U/μL
10X Reaction Buffer: 700mM Tris HCl (pH 7.6 at 25°C), 100mM MgCl2, 50mM DTT
Recombinant E. coli strain
7.5
100U
20mM Tris HCl (pH 7.5), 25mM KCl, 2mM DTT, 0.1mM EDTA, 0.1μM ATP, 50% Glycerol
Liquid
Colorless
Ammonium Ions and low levels of Phosphate Buffers
≥90%
132
5′ End-labeling of single or double-stranded DNA and RNA molecules for use as probes, sequencing or DNA Protein footprinting, Phosphorylation of DNA prior to cloning
−30 to −10°C
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