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Promega™ T4 DNA Ligase


Enzymes are available in convenient, cost-effective concentrations that are economical and easy to pipet

Manufacturer: promega™  M1801

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Catalog No. PR-M1801

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Description & Specifications

Specifications

Product Name T4 DNA Ligase
Format Liquid
Concentration 1 to 3U/μL
Color Colorless
Source Recombinant E. coli strain
Purity >90%
pH 7.4
Molecular Weight 68
Includes 10X Reaction Buffer: 300mM Tris HCl (pH 7.8 at 25°C), 100mM MgCl2, 100mM DTT and 10mM ATP
Quantity 100U
For Use With (Application) Joining double-stranded DNA molecules with cohesive or blunt ends (5', 3' or blunt-ended DNA inserts)
Storage Buffer 10mM Tris HCl (pH 7.4 at 25°C), 50mM KCl, 1mM DTT, 0.1mM EDTA, 50% Glycerol
Storage Requirements -20°C

T4 DNA Ligase catalyzes the joining of two strands of DNA between the 5'-phosphate and the 3'-hydroxyl groups of adjacent nucleotides in either a cohesive-ended or blunt-ended configuration. The enzyme has also been shown to catalyze the joining of RNA to either a DNA or RNA strand in a duplex molecule but will not join single-stranded nucleic acids.