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Promega™ SV Total RNA Isolation System


Membrane-based system for purification of intact total RNA from tissues, cultured cells, and white blood cells in as little as one hour

Manufacturer: promega™  Z3100

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Catalog No. PR-Z3100

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Description & Specifications

Specifications

For Use With (Application) Molecular biology applications, including RT-PCR and northern blotting
Format Spin column
Includes Collection tubes 2 x 25, Nuclease-free Water 1 x 13mL, elution tubes 2 x 25, RNA Lysis Buffer (RLA) 1 x 50mL, RNA dilution Buffer (RDA) 1 x 20mL, RNA wash solution (RWA) 1 x 58.8mL, DNase stop solution (DSA) 1 x 5.3mL, yellow core Buffer 1 x 2.5mL, MnCl2, 0.09M 1 x 250μl, DNase I (lyophilized) 1 x 1 vial, 2 x 25 spin columns, 1 x 2mL Beta-Mercaptoethanol
Quantity 50 Preps

  • Can process up to 60mg of tissue per purification, depending on tissue type
  • Incorporates a DNase treatment step directly on the minicolumn membrane that reduces genomic DNA contamination
  • Purification is acheved without using phenol:chloroform extractions or ethanol precipitations and there is no DNase carryover
  • Purified RNA obtained is suitable for all routine molecular biology applications, including RT-PCR and Northern blotting