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Promega™ SV Total RNA Isolation System DFS Item

A fast, simple technique for preparation of intact total RNA from tissues, cultured cells and white blood cells in as little as one hour.

Manufacturer:  Promega™ Z3100

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Catalog No. PR-Z3100

Includes: SV Total RNA Isolation System: Spin column assemblies and elution tubes, DNase I (lyophilized), SV RNA lysis buffer, SV RNA dilution buffer, β-mercaptoethanol, MnCl2 (0.09M), Yellow core buffer, SV DNase stop solution, SV RNA wash solution, Nuclease-free water
SV Total RNA Isolation Start-Up Kit (110V): SV Total RNA Isolation System, Vacuum Adapters, Welch™ Vacuum Pump for North America Electrical, Vac-Man™ Laboratory Vacuum Manifold (20-sample capacity)



  • Can process up to 60mg of tissue per purification, depending on tissue type
  • Incorporates a DNase treatment step directly on the minicolumn membrane that reduces genomic DNA contamination
  • Purification is acheved without using phenol:chloroform extractions or ethanol precipitations and there is no DNase carryover
  • Purified RNA obtained is suitable for all routine molecular biology applications, including RT-PCR and Northern blotting



Molecular biology applications, including RT-PCR and northern blotting
Collection tubes 2 x 25, Nuclease-free Water 1 x 13mL, elution tubes 2 x 25, RNA Lysis Buffer (RLA) 1 x 50mL, RNA dilution Buffer (RDA) 1 x 20mL, RNA wash solution (RWA) 1 x 58.8mL, DNase stop solution (DSA) 1 x 5.3mL, yellow core Buffer 1 x 2.5mL, MnCl2, 0.09M 1 x 250μl, DNase I (lyophilized) 1 x 1 vial, 2 x 25 spin columns, 1 x 2mL Beta-Mercaptoethanol
15 to 30°C
Spin column
50 Preps