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Promega™ SV Total RNA Isolation System DFS Item

Membrane-based system for purification of intact total RNA from tissues, cultured cells, and white blood cells in as little as one hour

Manufacturer:  Promega™ Z3380

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Catalog No. PRZ3380



Includes: SV Total RNA Isolation System: Spin column assemblies and elution tubes, DNase I (lyophilized), SV RNA lysis buffer, SV RNA dilution buffer, β-mercaptoethanol, MnCl2 (0.09M), Yellow core buffer, SV DNase stop solution, SV RNA wash solution, Nuclease-free water
SV Total RNA Isolation Start-Up Kit (110V): SV Total RNA Isolation System, Vacuum Adapters, Welch™ Vacuum Pump for North America Electrical, Vac-Man™ Laboratory Vacuum Manifold (20-sample capacity)

Description

Description

  • Can process up to 60mg of tissue per purification, depending on tissue type
  • Incorporates a DNase treatment step directly on the minicolumn membrane that reduces genomic DNA contamination
  • Purification is acheved without using phenol:chloroform extractions or ethanol precipitations and there is no DNase carryover
  • Purified RNA obtained is suitable for all routine molecular biology applications, including RT-PCR and Northern blotting

Specifications

Specifications

Molecular biology applications, including RT-PCR and northern blotting
Spin column
Spin column assemblies and elution tubes, DNase I (lyophilized), SV RNA Lysis Buffer, SV RNA dilution Buffer, beta-mercaptoethanol, MnCl2 (0.09M), yellow core Buffer, SV DNase stop solution, SV RNA wash solution, Nuclease-free Water
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