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Promega™ SV 96 Total RNA Isolation System DFS Item

The SV 96 Total RNA Isolation System provides a high-throughput technique to prepare intact RNA from tissue and cultured cells.

Manufacturer:  Promega™ Z3505

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Catalog No. PR-Z3505



Includes: Binding plate; DNA elution plate; DNase I (lyophilized); β-mercaptoethanol; SV DNase stop solution; MnCl2 (0.09M); Nuclease-free water; SV RNA wash solution; SV RNA lysis buffer; Yellow core buffer; Plate sealers

Requires : Use of the Vac-Man™ 96 Vacuum Manifold (Mfr. No. PR-A2291) or similar 96-well manifold for manual purification.

Description

Description

  • Substantially reduces genomic DNA contamination through a DNase treatment step
  • Purifies 96 samples at once, in less than one hour, with the walk-away automated protocol
  • Compatible with automated liquid-handling workstations
  • Eliminates the need for phenol/chloroform extraction or ethanol precipitation

Specifications

Specifications

Preparation of intact, purified total RNA and RT-PCR
Binding plate 5 x 1 plate, elution plate 5 x 1 plate, plate sealers 2 x 3, Nuclease-free Water 5 x 13mL, RNA Lysis Buffer (RLA) 2 x 50mL, RNA wash solution (RWA) 5 x 58.8mL, DNase stop solution (DSA) 5 x 13.25mL, yellow core Buffer 5 x 2.5mL, MnCl2, 0.09M 5 x 750μL, DNase I (lyophilized) 5 x 1 vial, Beta-Mercaptoethanol 2 x 2mL
5 x 96 Isolations
15 to 30°C
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