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Promega™ SoftLink™ Soft Release Avidin Resin


Provides reversible binding of biotinylated proteins under mild, nondenaturing conditions

Manufacturer: promega™  V2012

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Catalog No. PR-V2012

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Description & Specifications

Specifications

Flow Rate 300cm/hr.
For Use With (Application) Isolation and purification of Biotinylated Antibodies and other Proteins
pH Range 2 to 13
Product Type Avidin Resin
Quantity 5mL
Storage Requirements 4°C
Support Type Methacrylate Polymeric Gel filtration matrix

SoftLink Resin is a rigid, methacrylate polymeric gel filtration matrix, functionalized with covalently bound, monomeric avidin. Monomeric avidin allows both the specificity of capture and the mildness of release appropriate for the purification of sensitive biological materials.

  • Binds 20–40nmol of biotinylated protein per milliliter of resin
  • Retains biotin binding ability following exposure to a wide range of pH, low or high ionic strength solutions, 6M guanidine, and 1% SDS
  • Regenerates at least 10 times without loss of binding capacity
  • Supports high flowrates (300cm/hr.) and centrifugal forces (1500 × g) in batch applications
  • Ideal for the isolation and purification of biotinylated antibodies and other proteins