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Promega™ SignaTECT™ Protein Kinase Assay Systems


Contain proprietary SAM2 Biotin Capture Membrane for higher binding capacity and specificity

Manufacturer: promega™  V8161

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Catalog No. PR-V8161

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Description & Specifications

Specifications

Product Type Calcium/Calmodulin-dependent Protein Kinase assay
Quantity 96 Reactions
For Use With (Application) Can be used in a variety of Buffer or reaction conditions including cell extracts
Includes ATP, BSA, SAM2 Biotin capture membrane, Termination Buffer, CaM KII Biotinylated Peptide Substrate, reaction control, activation Buffer
Storage Requirements -20°C

  • Produces lower backgrounds and higher signal-to-noise ratios as compared to traditional P81 phosphocellulose methods
  • Perforated and numbered membrane allows measurement of up to 96 kinase reactions; ideal for kinetic studies
  • Assay requires little “hands-on” manipulation and is complete in under an hour
  • Can be used in a variety of buffer/reaction conditions including cell extracts
  • Each assay system contains highly specific biotinylated peptide substrates for the appropriate kinase as well as necessary reaction components
  • PTK assay system also contains two proprietary peptide substrates

User must supply [γ-32P] ATP