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Promega™ SignaTECT™ Protein Kinase Assay Systems DFS Item

Contain proprietary SAM2 Biotin Capture Membrane for higher binding capacity and specificity

Manufacturer:  Promega™ V7870

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Catalog No. PR-V7870



Description

Description

  • Produces lower backgrounds and higher signal-to-noise ratios as compared to traditional P81 phosphocellulose methods
  • Perforated and numbered membrane allows measurement of up to 96 kinase reactions; ideal for kinetic studies
  • Assay requires little “hands-on” manipulation and is complete in under an hour
  • Can be used in a variety of buffer/reaction conditions including cell extracts
  • Each assay system contains highly specific biotinylated peptide substrates for the appropriate kinase as well as necessary reaction components
  • PTK assay system also contains two proprietary peptide substrates

User must supply [γ-32P] ATP

U.S. Patents 6,066,462 and 6,348,310 and other patents pending.

Specifications

Specifications

DNA-dependent Protein Kinase assay system
Can be used in a variety of Buffer or reaction conditions including cell extracts
−30 to −10°C
96 Reactions
ATP, BSA, SAM2 Biotin capture membrane, Termination Buffer, CaM KII Biotinylated Peptide Substrate, reaction control, activation 5X Buffer
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