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Promega™ SGK1 Kinase Enzyme System DFS Item

Optimized for use with ADP-Glo™ Kinase Assay to measure the activity of human SGK1 kinase

$588.50 - $588.50


Molecular Weight 73
Storage Requirements -20° to -70°C
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Includes:  SGK1 Kinase Assay:
  • Kinase: Recombinant Human SGK1, 10μg (amino acids 60 to end), MW: ≅73kDa
  • Substrate: Akt (PKB) substrate (CKRPRAASFAE)
  • Other: Reaction Buffer, DTT
ADP-Glo Kinase Assay: Reagent, buffer, substrate, Ultra Pure ATP (10mM), ADP (10mM)
Catalog Number Mfr. No. For Use With (Application) Quantity Price Quantity    


DFS Item
For both primary screening as well as Kinase selectivity profiling, promotes cell survival by Phosphorylating and inactivating FKHRL1 10μg Each for $588.50
Description & Specifications


Molecular Weight 73
Storage Requirements -20° to -70°C

SGK1 is a member of the serum- and glucocorticoid-induced protein kinase family that is activated in vitro by 3-phosphoinositide-dependent protein kinase-1 (PDK1) and in vivo in response to signals that activate phosphatidylinositol (PI) 3-kinase. SGK1 mRNA is expressed in all tissues, and the level of SGK1 mRNA is increased by stimulation with serum or dexamethasone.

ADP-Glo Kinase Assay:

  • Luminescent assay that measures ADP formed from a kinase reaction
  • ADP is converted into ATP, which is a substrate in a reaction catalyzed by Ultra-Glo™ Luciferase that produces light
  • Luminescent signal positively correlates with ADP amount and kinase activity

Upon receipt, centrifuge the kinase and dispense it into smaller quantities.

ADP-Glo Kinase Assay: For both primary screening as well as kinase selectivity profiling