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Promega™ SGK1 Kinase Enzyme System


Optimized for use with ADP-Glo™ Kinase Assay to measure the activity of human SGK1 kinase

Manufacturer: promega™  V2911

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Catalog No. PRV2911

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Description & Specifications

Specifications

Molecular Weight 73
For Use With (Application) For both primary screening as well as Kinase selectivity profiling, promotes cell survival by Phosphorylating and inactivating FKHRL1
Quantity 10μg
Storage Requirements -20° to -70°C

SGK1 is a member of the serum- and glucocorticoid-induced protein kinase family that is activated in vitro by 3-phosphoinositide-dependent protein kinase-1 (PDK1) and in vivo in response to signals that activate phosphatidylinositol (PI) 3-kinase. SGK1 mRNA is expressed in all tissues, and the level of SGK1 mRNA is increased by stimulation with serum or dexamethasone.

ADP-Glo Kinase Assay:

  • Luminescent assay that measures ADP formed from a kinase reaction
  • ADP is converted into ATP, which is a substrate in a reaction catalyzed by Ultra-Glo™ Luciferase that produces light
  • Luminescent signal positively correlates with ADP amount and kinase activity

Upon receipt, centrifuge the kinase and dispense it into smaller quantities.

ADP-Glo Kinase Assay: For both primary screening as well as kinase selectivity profiling