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Promega™ Riboprobe™ Combination Systems

For in vitro prepartion of high specific activity single-stranded RNA probes or microgram quantities of defined RNA transcripts from cloned DNA inserts

Manufacturer: promega™  P1450

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Catalog No. PR-P1450


Description & Specifications


For Use With (Application) Generation of high-specific-activity RNA probes, generation of microgram quantities of RNA transcripts, generation of RNA processing substrates, generation of antisense RNA
Includes 500U T3 RNA Polymerase, 500U T7 RNA Polymerase, 5μg pGEM™ express positive control template, 500μL transcription optimized 5X Buffer, 2500U recombinant RNasin™ R
Product Type Riboprobe Combination System T3/T7
Quantity 1 System
Storage Requirements Store at -20°C.

  • All required reagents (excluding radioisotope) for performing transcription reactions in vitro and DNase to remove the template following transcription are included
  • SP6, T7 and T3 RNA Polymerases are extremely promoter-specific, allowing production of virtually homogeneous RNA using plasmid DNA as a template
  • Combination systems allow synthesis of RNA corresponding to either the coding or noncoding strand of cloned DNA from a single plasmid construct