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Promega Riboprobe™ Combination Systems DFS Item

The Riboprobe Systems are designed to prepare high-specific-activity single-stranded RNA probes or microgram quantities of defined RNA transcripts from cloned DNA inserts.

Manufacturer:  Promega P1460

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Catalog No. PR-P1460

Includes: RNA polymerase(s); pGEM™ Express Positive Control Template; RQ1 RNase-free DNase; Recombinant RNasin™ Ribonuclease Inhibitor; rATP; rCTP; rGTP; rUTP; DTT; Transcription-optimized buffer, 5X; Nuclease-free water



  • Produce High-Specific-Activity RNA Probes or Defined RNA Transcripts
  • Choose from combination systems with T3/T7 or SP6/T7 RNA polymerases
  • SP6, T7 and T3 RNA Polymerases are extremely promoter-specific, allowing production of virtually homogeneous RNA using plasmid DNA as a template
  • Allows synthesis of RNA corresponding to the coding or noncoding strand of cloned DNA from a single plasmid construct

U.S. Patent 5,552,302; Australian Patent 646803 and other patents.
Australian Patents 616881 and 641261 and other pending and issued patents, which claim vectors encoding a portion of human placental ribonuclease inhibitor, are exclusively licensed to Promega Corporation.



Generation of high-specific-activity RNA probes, generation of microgram quantities of RNA transcripts, generation of RNA processing substrates, generation of antisense RNA
Riboprobe Combination System SP6/T7
−30 to −10°C
500U SP6 RNA Polymerase, 500U T7 RNA Polymerase, 5μg pGEM™ express positive control template, 500μL transcription optimized 5X Buffer, 2500U recombinant RNasin™ R
1 System