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Promega™ Restriction Enzymes - Hae II (B) DFS Item

HaeII is a restriction enzymes (endonuclease) isolated from the Haemophilus aegyptius bacteria. Its recognition site is the GGCC nucleotide sequence.

Manufacturer:  Promega™ R6661

Catalog No. PR-R6661



Description

Description

For site-specific methylation information, consult McClelland, M., Nelson, M. and Raschke, E. (1994) Nucl. Acids Res. 22, 3640-59.

‡Restriction enzyme buffer included indicated in parentheses; Cloning = Blue/White Cloning Qualified; Genome = Genome Qualified.

Specifications

Specifications

HaeII (B)
10U/μL
1 x 1mL 10X Buffer B, 1 x 150μL Bovine Serum Albumin, Acetylate, 1 x 1000U HaeII, 1 x 0.25mL Multi-Core™ 10X Buffer
1000U
10mM Tris HCl (pH 7.4), 100mM KCl, 0.1mM EDTA, 1mM DTT, 0.5mg/mL BSA, 50% Glycerol
Liquid
(A/G)GCGC;(T/C)(T/C);CGCG(A/G)
37°C
Site-specific methylation
−30 to −10°C
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