Promega™ Restriction Enzymes - Bgl II (D)
Active and capable of digestion directly in GoTaq™ Green Master Mix. (A/GATC T T CTAG\A)
Manufacturer: Promega™ R6085
Ends generated with BglII can be directly ligated to ends generated with BamHI, BclI and XhoII. These restriction sites are not regenerated in the ligation product.
|1 x 1mL 10X Buffer D, 1 x 150μL Bovine Serum Albumin, Acetylated, 1 x 2500U BglII|
|Ends generated with BglII can be directly ligated to ends generated with BamHI, BclI and XhoII|
|−30 to −10°C|
|10mM Tris HCl (pH 7.3), 300mM NaCl, 0.1mM EDTA, 1mM DTT, 0.5mg/mL BSA, 50% Glycerol|