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Promega™ Regulated Mammalian Expression System


Offers low basal levels, robust and rapid induction, and downregulation of gene expression in mammalian cells

Manufacturer: promega™  C9421

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Catalog No. PRC9421

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Description & Specifications

Specifications

For Use With (Equipment) Regulated mammalian expression system and Flexi™ vector cloning system
For Use With (Application) Express a chimeric transactivator Protein that interacts with the regulatory promoter region in the pF12A and pF12K Flexi™ vectors in response to coumermycin and novobiocin, as well as encoding a Neomycin Phosphotransferase Gene that all
Marker Gene Hygromycin or Puromycin
Promoter SV40
Quantity 20μg
Vector pReg neo vector
Storage Requirements -20°C

The Regulated Mammalian Expression System is based on a novel on/off switch that relies on the rapid and sensitive modulation by coumerin-related compounds of a chimeric transactivator protein. Nanomolar concentrations of the antibiotic coumermycin promote homodimerization of a chimeric transactivator that, in turn, binds to lambda operator sequences located upstream of a minimal promoter driving transcription of coding sequences for a protein of interest. The levels of protein expression can be regulated by adjusting the coumermycin concentration and, more significantly, this expression can be switched off by adding novobiocin, which acts as an antagonist by dissociating the dimerized transactivator protein.

  • The pF12A or pF12K RM Flexi™ vectors are specially designed for regulated mammalian (RM) protein expression and incorporate regulatory promoter sequences upstream of the protein-coding region
  • The pReg neo vector is designed to express a chimeric transactivator protein that interacts with the regulatory promoter region in the pF12A and pF12K Flexi vectors in response to coumermycin and novobiocin, as well as encoding a neomycin phosphotransferase gene that allows stable cell selection and generation with the antibiotic G-418
  • Use of the pReg neo vector enables a high level of controlled induction combined with low basal protein expression
  • The co-transfection of the pF12A or pF12K RM Flexi vector containing the protein-coding region of interest and the pReg neo vector provides dose-response induction of protein expression, as well as rapid and sensitive on/off switch for protein expression