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Promega™ Regulated Mammalian Expression System

Offers low basal levels, robust and rapid induction, and downregulation of gene expression in mammalian cells

$300.04 - $949.06

Specifications

Quantity 20μg
Storage Requirements -20°C
View More Specs
Includes:  pReg neo Vector, 20μg; pF12A RM Flexi Vector, 20μg; pF12K RM Flexi Vector, 20μg. Vectors also available separately.

 Disclaimers

Patent Pending.
For research use only. Persons wishing to use this product or its derivatives in other fields of use, including without limitation, commercial sale, diagnostics or therapeutics, should contact Promega Corporation for licensing information.
For Nonprofit Entity research use only. Researchers at Nonprofit Entities may use this product in their own research and they may transfer derivatives of this product to others at Nonprofit Entities for research use provided that at the time of transfer a copy of this label license is given to the recipients and recipients agree to be bound by the terms and conditions of this label license. Researchers may not transfer this product or its derivatives to researchers at other entities that are not Nonprofit Entities (“For-profit Entities”) without the express written consent of Promega and without those For-profit Entities having an express license from Promega for the use of the product. For-profit Entities shall require an express license from Promega for any and all uses of the product or its derivatives. A Nonprofit Entity performing research for a For-profit Entity shall be considered a For-profit Entity for the purposes of this label license. With respect to manufacture or sale of research products, or any diagnostic, therapeutic or prophylactic uses, please contact Promega for supply and licensing information. No reach-through payments shall be owed to Promega relating to an entity's commercialization of products that are the discoveries resulting from research use of this product or its derivatives, provided that such products of the entity do not fall within the scope of the valid claims of any issued patents assigned or licensed to Promega, or that such commercialization would not be a violation of the terms and conditions of this label license. No other use or transfer of this product or its derivatives is authorized without the express, written consent of Promega. If the purchaser is not willing to accept the terms and conditions of this label license, Promega is willing to accept return of the unopened product and provide purchaser with a full refund. However, in the event that the product is opened, purchaser agrees to be bound by the terms and conditions of this label license.
Licensed from National Research Council of Canada.
The CMV promoter and its use are covered under U.S. Patents 5,168,062 and 5,385,839 owned by the University of Iowa Research Foundation, Iowa City, Iowa, and licensed for Research Use Only. Research Use includes contract research for which monetary or other consideration may be received. Other commercial users must obtain a license to these patents directly from the University of Iowa Research Foundation.

Products
Catalog Number Mfr. No. For Use With (Application) Quantity Vector Price Quantity    

PRC9421

 
promega™
C9421
Express a chimeric transactivator Protein that interacts with the regulatory promoter region in the pF12A and pF12K Flexi™ vectors in response to coumermycin and novobiocin, as well as encoding a Neomycin Phosphotransferase Gene that all 20μg pReg neo vector Each for $300.04

PRC9470

 
promega™
C9470
Regulated Protein expression in mammalian cells 20μg 20μg pReg neo vector, 20μg pF12A RM Flexi™ vector, 20μg pF12K RM Flexi™ vector Each for $949.06
Description & Specifications

Specifications

Quantity 20μg
Storage Requirements -20°C

The Regulated Mammalian Expression System is based on a novel on/off switch that relies on the rapid and sensitive modulation by coumerin-related compounds of a chimeric transactivator protein. Nanomolar concentrations of the antibiotic coumermycin promote homodimerization of a chimeric transactivator that, in turn, binds to lambda operator sequences located upstream of a minimal promoter driving transcription of coding sequences for a protein of interest. The levels of protein expression can be regulated by adjusting the coumermycin concentration and, more significantly, this expression can be switched off by adding novobiocin, which acts as an antagonist by dissociating the dimerized transactivator protein.

  • The pF12A or pF12K RM Flexi™ vectors are specially designed for regulated mammalian (RM) protein expression and incorporate regulatory promoter sequences upstream of the protein-coding region
  • The pReg neo vector is designed to express a chimeric transactivator protein that interacts with the regulatory promoter region in the pF12A and pF12K Flexi vectors in response to coumermycin and novobiocin, as well as encoding a neomycin phosphotransferase gene that allows stable cell selection and generation with the antibiotic G-418
  • Use of the pReg neo vector enables a high level of controlled induction combined with low basal protein expression
  • The co-transfection of the pF12A or pF12K RM Flexi vector containing the protein-coding region of interest and the pReg neo vector provides dose-response induction of protein expression, as well as rapid and sensitive on/off switch for protein expression