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Promega™ PureYield™ Plasmid Midiprep System


Isolates high-quality plasmid DNA from bacterial cultures

Manufacturer: promega™  A2492

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Catalog No. PR-A2492

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Description & Specifications

Specifications

For Use With (Application) Isolates high-quality plasmid DNA from bacterial cultures
Format Spin or vacuum
Includes 75mL Cell resuspension solution, 75mL Cell lysis solution, 130mL Neutralization solution, 85.3mL Endotoxin removal wash, 210mL Column wash, 25mL Nuclease-free water, 25 PureYield binding columns, 25 PureYield clearing columns
Product Type Plasmid Midiprep System
Quantity 25 Preps
Sample Size 50mL
Storage Requirements Store at 22° to 25°C.
Yield 100 to 200μg

  • Designed to purify 100 to 200μg of plasmid DNA from 50mL bacterial culture
  • May also be used with up to 250mL of bacterial culture using an alternative protocol
  • Utilizes a silica-membrane column which allows for the purification of large amounts of plasmid DNA
  • Incorporates an Endotoxin Removal Wash to remove substantial amounts of protein, RNA, and endotoxin contaminants
  • Compatible with a variety of protocols—centrifugation, vacuum, or a combination of centrifugation and vacuum
  • Provides rapid purification—no need for high-speed centrifugation, gravity-drip columns, or post-elution alcohol precipitation
  • Produces purified DNA suitable for sensitive applications such as eukaryotic transfection, in vitro transcription, and coupled in vitro transcription/translation