Promega Protein Deglycosylation Mix
Removes Glycans from both O-Linked and N-Linked glycosylation sites. Protein Deglycosylation Mix contains five protein deglycosidases, with fetuin provided as a deglycosylation substrate control.
Manufacturer: Promega V4931
Mixture of five (5) protein deglycosidases: PNGase F, O-Glycosidase, Neurominidase, β1-4 Galactosidase, β-N-Acetylglucosaminidase). Provides suffucient deglycosidase for 20 reactions.
|Protein Deglycosylation Mix|
|20mM Tris HCl (pH 7.5 at 25°C), 50mM NaCl, 5mM EDTA|
|100μL Protein Deglycosylation mix (PNGase F, O-Glycosidase, Neuraminidase, beta1-4 Galactosidase, beta-N-Acetylglucosaminidase), 1mL 10X Denaturing solution, 1mL 10X Deglycosylation Reaction Buffer (0.5M Sodium Phosphate (1X pH 7.5 at 25|
|Removing Glycans from both O-linked and N-linked glycosylation sites|
|2° to 10°C|