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Promega™ Proteasome-Glo™ Chymotrypsin-Like Cell-Based Assay System

Measures the chymotrypsin-like protease activity of the proteasome complex in cultured cells

Manufacturer: promega™  G8660

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Catalog No. PRG8660

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Description & Specifications


Format Liquid
Includes 1 x 50μL Suc LLVY Glo substrate, 1 x 10mL Proteasome Glo Cell based Buffer, 1 x 1 each Luciferin detection Reagent
pH 7.2
Quantity 10mL
Storage Requirements -20°C

Provides a luminogenic proteasome substrate, Suc-LLVY-amnioluciferin, in a buffer optimized for cell permeabilization, proteasome activity and luciferase activity. Addition of the Proteasome-Glo Cell-Based Assay Reagent in an add-mix-measure format results in proteasome cleavage of the substrate and rapid generation of a luminescent signal produced by the luciferase reaction.

  • Obtain activity data directly from a cellular environment Add-mix-measure protocol minimizes handling steps and makes the assays amenable to automation
  • Maximum sensitivity is reached 10 to 30 minutes after reagent addition
  • Avoids inherent fluorescent background signals, providing excellent signal-to-background readings
  • Assays are linear over three logs of cell number or four logs of proteasome concentration

Measuring the chymotrypsin-like activity of the proteasome in cultured cells; screening of inhibitor libraries and monitoring proteasome-regulated protein degradation in cells