Promega™ Proteasome-Glo™ Chymotrypsin-Like Cell-Based Assay System
The Proteasome-Glo™ Cell-Based Assays are homogeneous, luminescent assays that measure the chymotrypsin-like, trypsin-like and caspase-like protease activities associated with the proteasome complex in cultured cells.
Manufacturer: Promega™ G8660
Provides a luminogenic proteasome substrate, Suc-LLVY-amnioluciferin, in a buffer optimized for cell permeabilization, proteasome activity and luciferase activity. Addition of the Proteasome-Glo Cell-Based Assay Reagent in an add-mix-measure format results in proteasome cleavage of the substrate and rapid generation of a luminescent signal produced by the luciferase reaction.
- Obtain activity data directly from a cellular environment Add-mix-measure protocol minimizes handling steps and makes the assays amenable to automation
- Maximum sensitivity is reached 10 to 30 minutes after reagent addition
- Avoids inherent fluorescent background signals, providing excellent signal-to-background readings
- Assays are linear over three logs of cell number or four logs of proteasome concentration
Measuring the chymotrypsin-like activity of the proteasome in cultured cells; screening of inhibitor libraries and monitoring proteasome-regulated protein degradation in cells
U.S. Pat. No. 6,602,677, Australian Pat. No. 754312 and other patents and patents pending.
The method of recombinant expression of Coleoptera luciferase is covered by U.S. Patents 5,583,024; 5,674,713; and 5,700,673.
|−30 to −10°C|
|1 x 50μL Suc LLVY Glo substrate, 1 x 10mL Proteasome Glo Cell based Buffer, 1 x 1 each Luciferin detection Reagent|