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Promega™ ProFluor™ Tyrosine Phosphatase Assay Systems

Measures purified tyrosine phosphatase enzyme activity in a multiwell plate format and involves “add-mix-read” steps only

Manufacturer: promega™  V1281

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Catalog No. PR-V1281

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Description & Specifications


Format 96 or 384-well Plate
Product Type Tyrosine Phosphatase assay
Quantity 8 Plates
For Use With (Application) Measures the activity of purified Tyrosine Phosphatases in 96 or 384-well plates, ideal for high throughput applications
Storage Requirements -20°C

  • Ideal for high-throughput applications
  • Achieve highly predictive results: robust Z« values greater than 0.8 in either 96 or 384-well plate formats
  • Observe minimal test compound interference: substrate used at micromolar concentration, rhodamine 110 fluorescent signal produced is much higher than the fluorescent signal given off by test compounds
  • Control peptide included: control peptide (AAF-AMC) included that is used to monitor protease activity, reduces false positive hits
  • Simplify assays: simple add-mix-read format reduces the number of plate-handling steps to fewer than that required for other phosphatase assays
  • Save time: minimal throughput time compared to the multiple steps and lengthy incubations with other phoshatase assays
  • Non-radioactive: no radioactive waste disposal and safety issues

  • The assay produces excellent Z« values (>0.7) in either 96 or 384-well plate formats and easily distinguishes known phosphatase inhibitors from other compounds