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Promega™ ProFluor™ Src-Family Kinase Assay Systems DFS Item

Measures the activity of purified Src-family tyrosine kinases (Src, Lck, Lyn, Fyn, and Hck tested) in a multiwell plate format and involves “add-mix-read” steps only

Manufacturer:  Promega™ V1270

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Catalog No. PR-V1270



Description

Description

  • Ideal for high-throughput applications
  • Assay begins with a standard kinase reaction performed with a provided Src-family kinase bisamide rhodamine 110 peptide substrate fluorescence intensity measured in this assay is inversely correlated with kinase activity
  • A control peptide (AAF-AMC) is included to control for compounds that may inhibit the protease
  • The assay produces excellent Z« values (>0.7) in either 96 or 384-well plate formats and easily distinguishes known Src-family kinase inhibitors from other compounds
Specifications

Specifications

96 or 384-well Plate
4 Plates
−30 to −10°C
Src Family Protein Kinase assay
Measures the activity of purified Src-family Tyrosine Kinases in 96 or 384-well plates, ideal for high throughput applications
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