Please login to your online account to display your discounted pricing

Promega™ pRL Family of Renilla Luciferase Control Reporter Vectors

May be used in combination with a firefly luciferase vector to cotransfect mammalian cells

Manufacturer: promega™  E2231

 View all options for this product

Catalog No. PR-E2231


Description & Specifications


For Use With (Application) Provide constitutive expression of Renilla reniformis (sea pansy) Luciferase, can be used in combination with a firefly Luciferase vector to co-transfect mammalian cells
Promoter SV40
Reporter Gene Luciferase
Quantity 20μg
Vector pRL-SV40 vector
Storage Requirements -20°C
Storage Buffer TE Buffer (pH 7.4)

  • These vectors provide constitutive expression of Renilla reniformis (sea pansy) luciferase
  • Expression of Renilla luciferase provides an internal control value to which expression of the experimental firefly luciferase reporter gene may be normalized
  • A T7 promoter is located immediately upstream of Rluc, allowing in vitro synthesis of Renilla luciferase
  • The SV40 late poly(A) signal sequence is positioned downstream of Rluc to provide efficient transcription termination and mRNA polyadenylation
  • In addition to the modified Rluc reporter gene, all pRL Vectors are isolated from a dam–/dcmE. coli K host strain, allowing digestion with restriction enzymes that are sensitive to dam and dcm methylation
  • A prokaryotic origin of replication and β-lactamase gene allow selected propagation of the pRL vectors in E. coli host strains

  • Four different promoter configurations are available
  • The HSV-thymidine kinase promoter (pRL-TK) is relatively weak and may be particularly useful in providing neutral constitutive expression of the Renilla luciferase control reporter
  • The early SV40 enhancer/promoter region (pRL-SV40) and the CMV immediate early enhancer/promoter region (pRL-CMV) typically provide high-level transcription and, therefore, may be less suitable for coreporter applications involving experimental vectors with robust regulatory elements