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Promega™ PolyATtract™ mRNA Isolation Systems

Utilize MagneSphere™ technology to isolate poly(A)+ mRNA from total RNA in approximately 45 minutes

Manufacturer: promega™  Z5310

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Catalog No. PR-Z5310

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Description & Specifications


For Use With (Application) In-vitro translation, RT-PCR and cDNA synthesis
Includes Nuclease-free Water 2 x 25mL, SSC (20X) 2 x 1.4mL, Biotinylated Oligo(dT) probe 1 x 50μL, Streptavidin MagneSphere™ paramagnetic particles 15 x 0.6mL
Quantity 15 Preps

Use a biotinylated oligo(dT) primer to hybridize, at high efficiency in solution, to the 3« poly(A)+ region present in most mature eukaryotic mRNA species. The hybrids are bound to streptavidin coupled to paramagnetic particles, captured using a magnetic separation stand, and washed at high stringency. The mRNA is eluted from the solid phase with ribonuclease-free deionized water.

  • Produces mRNA that is suitable for all molecular biology applications, including in vitro translation, RT-PCR, and cDNA synthesis
  • Configurable for use with large or small amounts of cells and tissues
  • Ideal for use with robotic platforms