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Promega™ PolyATtract™ mRNA Isolation Systems DFS Item

The PolyATtract System isolates messenger RNA directly from total RNA.

Manufacturer:  Promega™ Z5210*

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Catalog No. PR-Z5210



Use a biotinylated oligo(dT) primer to hybridize, at high efficiency in solution, to the 3« poly(A)+ region present in most mature eukaryotic mRNA species. The hybrids are bound to streptavidin coupled to paramagnetic particles, captured using a magnetic separation stand, and washed at high stringency. The mRNA is eluted from the solid phase with ribonuclease-free deionized water.

  • Produces mRNA that is suitable for all molecular biology applications, including in vitro translation, RT-PCR, and cDNA synthesis
  • Configurable for use with large or small amounts of cells and tissues
  • Ideal for use with robotic platforms



In-vitro translation, RT-PCR and cDNA synthesis
Nuclease-free Water 3 x 25mL, SSC (20X) 3 x 1.4mL, Biotinylated Oligo(dT) probe 1 x 35μL, mRNA user tube 3 x 1, Streptavidin MagneSphere™ paramagnetic particles 3 x 3mL
3 Preps
2 to 10°C