Promega™ PolyATtract™ mRNA Isolation Systems
The PolyATtract System isolates messenger RNA directly from total RNA.
Manufacturer: Promega™ Z5210*
Use a biotinylated oligo(dT) primer to hybridize, at high efficiency in solution, to the 3« poly(A)+ region present in most mature eukaryotic mRNA species. The hybrids are bound to streptavidin coupled to paramagnetic particles, captured using a magnetic separation stand, and washed at high stringency. The mRNA is eluted from the solid phase with ribonuclease-free deionized water.
- Produces mRNA that is suitable for all molecular biology applications, including in vitro translation, RT-PCR, and cDNA synthesis
- Configurable for use with large or small amounts of cells and tissues
- Ideal for use with robotic platforms
|In-vitro translation, RT-PCR and cDNA synthesis|
|Nuclease-free Water 3 x 25mL, SSC (20X) 3 x 1.4mL, Biotinylated Oligo(dT) probe 1 x 35μL, mRNA user tube 3 x 1, Streptavidin MagneSphere™ paramagnetic particles 3 x 3mL|
|2 to 10°C|