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Promega™ Pgp-Glo™ Assay System


Detects effects of compounds on recombinant human P-glycoprotein (Pgp) in a cell membrane fraction

Manufacturer: promega™  V3591

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Catalog No. PRV3591

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Description & Specifications

Specifications

Format Fluid
Includes 1 x 10mL Pgp Glo Assay Buffer, 1 x 10mL ATP detection Buffer, 1 x 1 vial ATP detection substrate, 1 x 100μL Verapamil, 1 x 1mL MgATP, 1 x 250μL Sodium Vanadate
pH 6
Quantity 10mL
Storage Requirements -70°C

Assay1 relies on ATP dependence of light-generating reaction of firefly luciferase. Proprietary stabilized firefly luciferase and proprietary luciferase assay formulation to minimize incidence of false positives.

  • Provides necessary reagents to perform luminescent P-glycoprotein (Pgp) ATPase assays
  • Stable glow-type signal allows for multiple sample processing without concerns of variability over time
  • Low false-positive rate
  • Simple protocol makes assay suitable for high-throughput screening in multiwell plates

Screening drugs and new chemical entities for capacity to modulate Pgp ATPase activity