Promega™ Pgp-Glo™ Assay System
The Pgp-Glo™ Assay Systems provide the necessary reagents for performing luminescent P-glycoprotein (Pgp) ATPase assays.
Manufacturer: Promega™ V3591
Assay1 relies on ATP dependence of light-generating reaction of firefly luciferase. Proprietary stabilized firefly luciferase and proprietary luciferase assay formulation to minimize incidence of false positives.
- Provides necessary reagents to perform luminescent P-glycoprotein (Pgp) ATPase assays
- Stable glow-type signal allows for multiple sample processing without concerns of variability over time
- Low false-positive rate
- Simple protocol makes assay suitable for high-throughput screening in multiwell plates
Screening drugs and new chemical entities for capacity to modulate Pgp ATPase activity
|−30 to −10°C|
|1 x 10mL Pgp Glo Assay Buffer, 1 x 10mL ATP detection Buffer, 1 x 1 vial ATP detection substrate, 1 x 100μL Verapamil, 1 x 1mL MgATP, 1 x 250μL Sodium Vanadate|
1The method of recombinant expression of Coleoptera luciferase is covered by U.S. Pat. Nos. 5,583,024, 5,674,713 and 5,700,673.