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Promega™ Pgp-Glo™ Assay System DFS Item

Detects effects of compounds on recombinant human P-glycoprotein (Pgp) in a cell membrane fraction

$399.11 - $798.21

Specifications

Format Fluid
Quantity 10mL
Storage Requirements -70°C
View More Specs

Disclaimers:

1The method of recombinant expression of Coleoptera luciferase is covered by U.S. Pat. Nos. 5,583,024, 5,674,713 and 5,700,673.



Products ${productFamilyLength}
Catalog Number Mfr. No. Includes pH Price Quantity  
Catalog Number Mfr. No. Includes pH Price Quantity  
PRV3591 Promega™
V3591
1 x 10mL Pgp Glo Assay Buffer, 1 x 10mL ATP detection Buffer, 1 x 1 vial ATP detection substrate, 1 x 100μL Verapamil, 1 x 1mL MgATP, 1 x 250μL Sodium Vanadate 6 Each for $399.11
PRV3601 Promega™
V3601
1 x 10mL Pgp Glo Assay Buffer, 1 x 10mL ATP detection Buffer, 1 x 1 vial ATP detection substrate, 1 x 0.5mL Human Pgp Membranes recombinant, 1 x 100μL Verapamil, 1 x 1mL MgATP, 1 x 250μL Sodium Vanadate 6.8 Each for $798.21
Specifications

Description

Assay1 relies on ATP dependence of light-generating reaction of firefly luciferase. Proprietary stabilized firefly luciferase and proprietary luciferase assay formulation to minimize incidence of false positives.

  • Provides necessary reagents to perform luminescent P-glycoprotein (Pgp) ATPase assays
  • Stable glow-type signal allows for multiple sample processing without concerns of variability over time
  • Low false-positive rate
  • Simple protocol makes assay suitable for high-throughput screening in multiwell plates

Screening drugs and new chemical entities for capacity to modulate Pgp ATPase activity

Description & Specifications

Specifications

Fluid
10mL
-70°C