Please login to your online account to display your discounted pricing

Promega™ Pgp-Glo™ Assay System

Detects effects of compounds on recombinant human P-glycoprotein (Pgp) in a cell membrane fraction

$386.27 - $771.47

Specifications

Format Fluid
Quantity 10mL
Storage Requirements -70°C
View More Specs

 Disclaimers

1The method of recombinant expression of Coleoptera luciferase is covered by U.S. Pat. Nos. 5,583,024, 5,674,713 and 5,700,673.

Products
Catalog Number Mfr. No. Includes pH Price Quantity    

PRV3591

 
promega™
V3591
1 x 10mL Pgp Glo Assay Buffer, 1 x 10mL ATP detection Buffer, 1 x 1 vial ATP detection substrate, 1 x 100μL Verapamil, 1 x 1mL MgATP, 1 x 250μL Sodium Vanadate 6 Each for $386.27

PRV3601

 
promega™
V3601
1 x 10mL Pgp Glo Assay Buffer, 1 x 10mL ATP detection Buffer, 1 x 1 vial ATP detection substrate, 1 x 0.5mL Human Pgp Membranes recombinant, 1 x 100μL Verapamil, 1 x 1mL MgATP, 1 x 250μL Sodium Vanadate 6.8 Each for $771.47
Description & Specifications

Specifications

Format Fluid
Quantity 10mL
Storage Requirements -70°C

Assay1 relies on ATP dependence of light-generating reaction of firefly luciferase. Proprietary stabilized firefly luciferase and proprietary luciferase assay formulation to minimize incidence of false positives.

  • Provides necessary reagents to perform luminescent P-glycoprotein (Pgp) ATPase assays
  • Stable glow-type signal allows for multiple sample processing without concerns of variability over time
  • Low false-positive rate
  • Simple protocol makes assay suitable for high-throughput screening in multiwell plates

Screening drugs and new chemical entities for capacity to modulate Pgp ATPase activity